Product Description

ATG4D Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61625-50, BS61625-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Autophagy, a process that resμlts in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagyrelated gene (Atg) proteins. Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease that is required for autophagy and functions to specifically cleave the C-terminal region of target proteins, thereby allowing the target proteins to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by N-ethylmaleimide, a thiol reactive compound that is capable of modifying cystine residues in proteins and peptides.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, mitochondrial, ATG4D, APG4D, AUTL4

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding Human ATG4D.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ATG4D polyclonal antibody detects endogenous levels of ATG4D protein.

Molecular Weight: ~ 52 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q86TL0

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only