Product Description

ATP/ADP Ratio Chemiluminescence Assay kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F004-48, E-BC-F004-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Adenosine triphosphate (ATP) is the energy currency of organisms, produced by glycolysis or the tricarboxylic acid cycle and electron transport chain in mitochondria, and is the source of energy in cells. Under normal circumstances, ATP and adenosine diphosphate (ADP) will convert to each other, and the change of the ratio of ATP to ADP is closely related to apoptosis, autophagy, energy metabolism and other pathways, so it is often used as an indicator of cell activity.
Under the catalysis of luciferase, ATP react with luciferin and emits fluorescence. Convert ADP to ATP by enzyme, and ATP was detected by the same principle. The ATP/ADP ratio in the cell can be calculated.

Research Areas: Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Quantitative

Detection Instrument: Chemiluminescence immunoassay analyzer;multifunctional microplate reader (with chemiluminescence)

Detection Type: Fluorometric method

Tested Sample Types: Cell

Assay Length: 1 h

Inter cv: 2.9-9.4%

Intra cv: 3.5-7.6%

Research Use Only