Product Description

ATP Colorimetric Assay Kit (Enzyme Method)

Sizes: 96T

Catalogue Numbers: E-BC-K774-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Adenosine Triphosphate (ATP) is a high-energy phosphate compound, which is the most direct energy source in the body, and its content is directly related to the state of energy metabolism in the body. Hexokinase (HK) catalyzes the generation of glucose and ATP into glucose-6-phosphate, and glucose-6-phosphate dehydrogenase (G6PD) further catalyzes the dehydrogenation of glucose-6-phosphate into NADPH. NADPH can react with the color developer, and the product has a characteristic absorption peak at 460 nm to determine the ATP content.

Abbreviation: ATP

Research Areas: Tricarboxylic Acid（TCA）Cycle;Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.72-40.00 μmol/L

Tested Sample Types: Animal tissue

Assay Length: 40 min

Sensitivity: 0.72 μmol/L

Inter cv: 1.7-6.4%

Intra cv: 1.5-2.2%

Research Use Only