Product Description

BART1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1750-50, ES1750-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: ARL2BP, BART, BART1, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, Binder of ARF2 protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ARL2BP. AA range:101-150

Isotype: IgG

Target: BART1

Specificity: BART1 Polyclonal Antibody detects endogenous levels of BART1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in retina pigment epithelial cells (at protein level), Widely expressed,

Observed Band (KD): 25kD

Function: Function: May play a role as an effector of the ADP-ribosylation factor-like protein 2, ARL2
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Belongs to the ARL2BP family
Subunit: Interacts with GTP bound ARL2 and ARL3, the complex ARL2-ARL2BP as well as ARL2BP alone, binds to ANT1
Tissue Specificity: Ubiquitous

Subcellular Location: Cytoplasm Mitochondrion intermembrane space Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nucleus Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, cilium basal body The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity) Detected in the midbody matrix Not detected in the Golgi, nucleus and on the mitotic spindle Centrosome-associated throughout the cell cycle Not detected to interphase microtubules In retina photoreceptor cells, localized in the distal connecting cilia, basal body, ciliary-associated centriole, and ciliary rootlet Interaction with ARL2 may be required for cilia basal body localization

Storage and Stability: -20°C/1 year

Gene Name: ARL2BP

Protein Name: ADP-ribosylation factor-like protein 2-binding protein

Mouse Gene ID: 107566

Mouse SwissProt Number: Q9D385

Rat Gene ID: 498910

Rat SwissProt Number: Q4V8C5

Research Use Only