Product Description

Bax (phospho Thr167) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6946-50, ES6946-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, ELISA

Alternate Names: BAX, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Thr167. AA range:133-182

Isotype: IgG

Target: Bax

Specificity: Phospho-Bax (T167) Polyclonal Antibody detects endogenous levels of Bax protein only when phosphorylated at T167.

Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in a wide variety of tissues, Isoform Psi is found in glial tumors, Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung, Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin, Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines,

Molecular Weight (Da): 21kD

Fields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, Platinum drug resistance, Sphingolipid signaling pathway, p53 signaling pathway, Protein processing in endoplasmic reticulum, Apoptosis, Longevity regulating pathway, Apoptosis - multiple species, Necroptosis, Neurotrophin signaling pathway, Non-alcoholic fatty liver disease, AGE-RAGE signaling pathway in diabetic complications, Parkinson disease, Amyotrophic lateral sclerosis, Huntington disease, Prion disease, Pathways of neurodegeneration - multiple diseases, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection, Tuberculosis, Hepatitis C, Hepatitis B, Measles, Human cytomegalovirus infection, Influenza A, Human papillomavirus infection, Human T-cell leukemia virus 1 infection, Kaposi sarcoma-associated herpesvirus infection, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Human immunodeficiency virus 1 infection, Pathways in cancer, Transcriptional

Function: Disease: Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma
Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family
Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis
Similarity: Belongs to the Bcl-2 family
Subcellular Location: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, redistributes to the mitochondrion membrane through the release from JNK-phosphorylated 14-3-3 proteins
Subunit: Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1

Subcellular Location: [Isoform Alpha]: Mitochondrion outer membrane, Single-pass membrane protein Cytoplasm Colocalizes with 14-3-3 proteins in the cytoplasm Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane Upon Sendai virus infection, recruited to the mitochondrion through interaction with IRF3 (PubMed:25609812), [Isoform Beta]: Cytoplasm, [Isoform Gamma]: Cytoplasm, [Isoform Delta]: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: BAX

Protein Name: Apoptosis regulator BAX

Mouse Gene ID: 12028

Mouse SwissProt Number: Q07813

Rat SwissProt Number: Q63690

Research Use Only