Product Description

BH2 (Dihydrobiopterin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9938-48, ELK9938-96, ELK9938-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BH2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Dihydrobiopterin

Reactivity: General

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction

Assay Length: 3.5h

Sensitivity: 11.31 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Research Use Only