Product Description

BIG2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1770-50, ES1770-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: ARFGEF2, ARFGEP2, BIG2, Brefeldin A-inhibited guanine nucleotide-exchange protein 2, Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540

Isotype: IgG

Target: BIG2

Specificity: BIG2 Polyclonal Antibody detects endogenous levels of BIG2 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in placenta, lung, heart, brain, kidney and pancreas,

Observed Band (KD): 210kD

Fields: Endocytosis

Function: Disease: Defects in ARFGEF2 are the cause of autosomal recessive periventricular nodular heterotopia type 2 (PVNH2) [MIM: 608097], also called periventricular heterotopia with microcephaly autosomal recessive. PVNH2 is an autosomal recessive form characterized by microcephaly (small brain), severe developmental delay and recurrent infections. No anomalies extrinsic to the central nervous system, such as dysmorphic features or grossly abnormal endocrine or other conditions, are associated with PVNH2
Enzyme Regulation: Inhibited by brefeldin A
Function: Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Contains 1 SEC7 domain
Tissue Specificity: Expressed in placenta, lung, heart, brain, kidney and pancreas

Subcellular Location: Cytoplasm Membrane Golgi apparatus Cytoplasm, perinuclear region Golgi apparatus, trans-Golgi network Endosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cell projection, dendrite Cytoplasmic vesicle Cell junction, synapse Cytoplasm, cytoskeleton Translocates from cytoplasm to membranes upon cAMP treatment Localized in recycling endosomes

Storage and Stability: -20°C/1 year

Gene Name: ARFGEF2

Protein Name: Brefeldin A-inhibited guanine nucleotide-exchange protein 2

Mouse Gene ID: 99371

Mouse SwissProt Number: A2A5R2

Rat Gene ID: 296380

Rat SwissProt Number: Q7TSU1

Research Use Only