Product Description

Biotin ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10918-48, ELK10918-96, ELK10918-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: General

Alternate Names: Biotin

Sensitivity: 4.7 pg/mL

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 2h

Research Area: Enzyme And Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only