Product Description

Brk (phospho Tyr447) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6916-50, ES6916-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: protein tyrosine kinase 6(PTK6) Homo sapiens The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: PTK6, BRK, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Breast Tumor Kinase around the phosphorylation site of Tyr447. AA range:402-451

Isotype: IgG

Target: Brk

Specificity: Phospho-Brk (Y447) Polyclonal Antibody detects endogenous levels of Brk protein only when phosphorylated at Y447.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Epithelia-specific, Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues, Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers, Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers, Also found in melanocytes, Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines,

Observed Band (KD): 50kD

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Enzyme Regulation: Activated enzyme seems to have greater access to its substrates
Function: Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression
PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 SH2 domain
Similarity: Contains 1 SH3 domain
Subcellular Location: Colocalizes with KHDRBS1, KHDRBS2 o

Subcellular Location: Cytoplasm Nucleus Cell projection, ruffle Membrane Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus Nuclear localization in epithelial cells of normal prostate but cytoplasmic localization in cancer prostate

Storage and Stability: -20°C/1 year

Gene Name: PTK6

Protein Name: Protein-tyrosine kinase 6

Mouse SwissProt Number: Q64434

Research Use Only