Product Description

BUB1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7356-50, ES7356-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

Isotype: IgG

Target: BUB1

Specificity: BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: High expression in testis and thymus, less in colon, spleen, lung and small intestine, Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus, Expression is associated with cells/tissues with a high mitotic index,

Observed Band (KD): 120kD

Fields: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Disease: Defects in BUB1 are associated with tumor formation
Domain: CD1 domain directs kinetochore localization and binding to BUB3
Enzyme Regulation: Autophosphorylated when the cells enters mitosis
Function: Involved in cell cycle checkpoint enforcement. Can interact and phosphorylate BUB3
Induction: Inhibited by phorbol 12-myristate 13-acetate (PMA)
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily
Similarity: Contains 1 CD1 domain
Similarity: Contains 1 protein kinase domain
Subcellular Location: Nuclear in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage
Tissue Specificity: High expressio

Subcellular Location: Nucleus Chromosome, centromere, kinetochore Nuclear in interphase cells Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis Localizes to the outer kinetochore Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: BUB1

Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1

Mouse SwissProt Number: O08901

Research Use Only