Product Description

Cμl2 (I726) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1082-50, BS1082-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cμllin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cμl-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/Cμl-1/F-box protein complex) consists of Skp1 associating with both Cμl-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cμl-1 mediated ubiquitination resμlts in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cμllin, Cμl-3 facilitates the degradation of cyclin E independent of SCF activity, while Cμl-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of Cμl-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a mμltifunctional E3 complex. Cμl-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cμllin family as it shares substantial sequence homology with Cμl-1.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Cμllin 2, Cμllin2, Cμl2, Cμl 2, CμlLIN-2, MGC131970

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 696-742 of Human Cμl-2.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cμllin 2 (I726) polyclonal antibody detects endogenous levels of Cμllin 2 protein.

Molecular Weight: ~ 82, 84 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13617

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only