Product Description

Cμllin 2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS6991-50, BS6991-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Core component of mμltiple cμllin-RING-based ECS (ElonginB/C-Cμl2/5-SOCS-box protein E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. Cμl2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cμllin subunit and is inhibited by the association of the deneddylated cμllin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL mediates the ubiquitination of hypoxia-inducible factor (HIF. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component are part of the DesCEND (destruction via C-end degrons pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: Cμl2, cμllin-2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human Cμllin 2(NP_001185708.1).

Dilution: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Molecular Weight: 87kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13617

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only