Product Description

C/EBP α (phospho Thr230) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4488-50, ES4488-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha

Clonality: Polyclonal

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human C/EBP α (phospho Thr230)

Isotype: IgG

Target: C/EBP α

Specificity: Phospho-C/EBP α (T230) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T230.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Liver, Pancreas, Umbilical cord, White Matter pool- 5 brain tissues- f

Observed Band (KD): 42,also have 30kd isform

Fields: Non-alcoholic fatty liver disease, Pathways in cancer, Transcriptional misregulation in cancer, Acute myeloid leukemia

Function: Function: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers
Similarity: Belongs to the bZIP family
Similarity: Belongs to the bZIP family. C/EBP subfamily
Similarity: Contains 1 bZIP domain
Subunit: Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X

Subcellular Location: Nucleus, [Isoform 4]: Nucleus, nucleolus

Storage and Stability: -20°C/1 year

Gene Name: CEBPA

Protein Name: CCAAT/enhancer-binding protein alpha

Mouse Gene ID: 12606

Mouse SwissProt Number: P53566

Rat Gene ID: 24252

Rat SwissProt Number: P05554

Research Use Only