Product Description

C/EBP α (phospho-S21) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4217-50, BS4217-100

Swiss-Prot: P49715

Host: Rabbit

C/EBP α (phospho-S21) Polyclonal Antibody-BS4217

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-C/EBP α (S21) polyclonal antibody detects endogenous levels of C/EBP α protein only when phosphorylated at Ser21.

Bioworld Molecular Weight: ~ 30,45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C/EBP α (phospho-S21) polyclonal antibody at 1:500 dilution
Lane 1: L02 whole cell lysate
Lane 2: L02 treated with PBS (1×PBS,PH7.4) for 1 houre then treated with DMEM (10%FBS) for 5 minutes whole cell lysate
Lane 3: L02 treated with PBS (1×PBS,PH7.4) for 1 houre then treated with DMEM (10%FBS) for 10 minutes whole cell lysate
Lane 4: L02 treated with PBS (1×PBS,PH7.4) for 1 houre then treated with DMEM (10%FBS) for 15 minutes whole cell lysate, Immunohistochemistry (IHC) analyzes of p-C/EBP α (S21) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Alternative Name: CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; C/EBP α; C/EBPα

Immunogen: Synthetic phosphopeptide derived from human C/EBP α around the phosphorylation site of Serine 21.

Modification: Phosphorylation

Conjugate: Unconjugated