Product Description

C/EBP ζ (D24) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2297-50, BS2297-100

Sizes: 50µl, 100µl

Swiss-Prot: Q03701

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: C/EBP family members include C/EBP α, C/EBP β, C/EBP γ, C/EBP δ, C/EBP ε and C/EBP ζ, all of which are capable of forming homodimers or heterodimers with each other. C/EBP ζ, also known as CBF2, NOC1, CHOP10 (C/EBP homologous protein 10), GADD153 (growth arrest and DNA damage-inducible gene 153) or HSPCBF, is a ubiquitously expressed 1,054 amino acid protein that localizes to the nucleus and regulates transcription. More specifically, C/EBP ζ is believed to play a role regulating the expression of CRP (C-reactive protein). Unlike most C/EBP dimers, C/EBP ζ-containing dimers do not bind the classical C/EBP-binding consensus sequence. Rather, C/EBP ζ-containing dimers must associate with RBP-Jκ (another transcription factor) in order to accomplish binding to the C/EBP site.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: C/EBP ζ (D24) polyclonal antibody detects endogenous levels of C/EBP ζ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C/EBP ζ (D24) pAb at 1:500 dilution
Lane 1: PC12 whole cell lysate (40ug)
Lane 2: 3T3-L1 whole cell lysate (40ug)
Lane 3: A2780 whole cell lysate (40ug)
Lane 4: Hela whole cell lysate (40ug)
Lane 5: MCF-7 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of C/EBP ζ (D24) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~121 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CBF2; CCAAT binding factor; CCAAT box binding transcription factor; CCAAT/enhancer binding protein zeta; Cebpz; HSPCBF; CBF;

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human C/EBP ζ.

Conjugate: Unconjugated

Modification: Unmodified