Product Description

CA IX Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1812-50, ES1812-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CA9, G250, MN, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CA IX. AA range:33-82

Isotype: IgG

Target: CA IX

Specificity: CA IX Polyclonal Antibody detects endogenous levels of CA IX protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed primarily in carcinoma cells lines, Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa,

Observed Band (KD): 58kD

Fields: Nitrogen metabolism, Metabolic pathways

Function: Catalytic Activity: H(2)CO(3) = CO(2) + H(2)O
Cofactor: Zinc
Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia
Induction: By hypoxia
PTM: Asn-346 bears high-mannose type glycan structures
Similarity: Belongs to the alpha-carbonic anhydrase family
Subcellular Location: Found on the surface microvilli and in the nucleus, particularly in nucleolus
Subunit: Forms oligomers linked by disulfide bonds
Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa

Subcellular Location: Nucleus Nucleus, nucleolus Cell membrane, Single-pass type I membrane protein Cell projection, microvillus membrane, Single-pass type I membrane protein Found on the surface microvilli and in the nucleus, particularly in nucleolus

Storage and Stability: -20°C/1 year

Gene Name: CA9

Protein Name: Carbonic anhydrase 9

Mouse SwissProt Number: Q8VHB5

Research Use Only