Product Description

CacyBP Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4119-50, ES4119-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CACYBP, S100A6BP, SIP, PNAS-107, Calcyclin-binding protein, CacyBP, hCacyBP, S100A6-binding protein, Siah-interacting protein

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human CACYBP. AA range:171-220

Isotype: IgG

Target: CacyBP

Specificity: CacyBP Polyclonal Antibody detects endogenous levels of CacyBP protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Colon carcinoma, Hepatoma, Liver, Promyelocytic leukemia, Skeletal muscle

Observed Band (KD): 25kD

Fields: Wnt signaling pathway

Function: Function: May be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1)
PTM: Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations
Similarity: Contains 1 CS domain
Similarity: Contains 1 SGS domain
Subcellular Location: Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated
Subunit: Interacts with protein of the S100 family S100A1, S100A6, S100B, S100P and S100A12 at physiological calcium concentrations (By similarity). Component of some large E3 complex at least composed of UBE2D1, SIAH1, CAC

Subcellular Location: Nucleus Cytoplasm Cytoplasmic at low calcium concentrations In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm The nuclear fraction may be phosphorylated

Storage and Stability: -20°C/1 year

Gene Name: CACYBP

Protein Name: Calcyclin-binding protein

Mouse Gene ID: 12301

Mouse SwissProt Number: Q9CXW3

Rat Gene ID: 289144

Rat SwissProt Number: Q6AYK6

Research Use Only