Caffeine ELISA Kit - ELK0683

Caffeine ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0683-48, ELK0683-96, ELK0683-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Caffeine

Assay Type: Competitive Inhibition

Sensitivity: 0.25 ng/mL

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2.5h

Research Areas: Signal transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Caffeine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caffeine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caffeine in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only