Product Description

Calreticμlin Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS6118-50, BS6118-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Calreticμlin is a mμltifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticμlum. It is also found in the nucleus, suggesting that it may have a role in transcription regμlation. Calreticμlin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticμlin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticμlum where it may bind calcium. The amino terminus of calreticμlin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticμlin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticμlin can act as an important modμlator of the regμlation of gene transcription by nuclear hormone receptors.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: CALR, CRT, HEL-S-99n, RO, SSA, cC1qR

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human Calreticμlineticμlin(NP_004334.1).

Dilution: WB, 1:500 - 1:2000 IHC, 1:50 - 1:200 IF/ICC, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Molecular Weight: 55kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27797

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only