Product Description

CaMKIV Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7636-50, ES7636-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CAMK4, CAMK, CAMK-GR, CAMKIV, Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CaMK4. AA range:166-215

Isotype: IgG

Target: CaMKIV

Specificity: CaMKIV Polyclonal Antibody detects endogenous levels of CaMKIV protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue,

Observed Band (KD): 60kD

Fields: Calcium signaling pathway, cAMP signaling pathway, Longevity regulating pathway, Apelin signaling pathway, Osteoclast differentiation, Long-term potentiation, Neurotrophin signaling pathway, Cholinergic synapse, Oxytocin signaling pathway, Aldosterone synthesis and secretion, Amphetamine addiction, Alcoholism, Glioma

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Enzyme Regulation: Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A
Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role i

Subcellular Location: Cytoplasm Nucleus Localized in hippocampal neuron nuclei In spermatids, associated with chromatin and nuclear matrix (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: CAMK4

Protein Name: Calcium/calmodulin-dependent protein kinase type IV

Mouse SwissProt Number: P08414

Rat Gene ID: 25050

Rat SwissProt Number: P13234

Research Use Only