Casein Kinase Iε (P301) Polyclonal Antibody-BS2279

Casein Kinase Iε (P301) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2279-50, BS2279-100

Sizes: 50µl, 100µl

Swiss-Prot: P49674

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, γ, ε and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The α catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Casein Kinase Iε (P301) polyclonal antibody detects endogenous levels of Casein Kinase Iε protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Casein Kinase Iε (P301) pAb at 1:500 dilution
Lane 1: 3T3-L1 whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug)
Lane 5: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Casein Kinase Iε (P301) pAb in paraffin-embedded human placenta tissue.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Casein kinase I isoform epsilon; CKI-epsilon; CKIe; CSNK1E; CKIE; CK-IE;

Immunogen: Synthetic peptide, corresponding to amino acids 250-350 of Human Casein Kinase Iε.

Conjugate: Unconjugated

Modification: Unmodified