Cat SDMA (Symmetric dimethylarginine) ELISA Kit - ELK9318

Cat SDMA (Symmetric dimethylarginine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9318-48, ELK9318-96, ELK9318-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Symmetric dimethylarginine

Assay Type: Competitive Inhibition

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Developmental science; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cat SDMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cat SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cat SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only