Catenin-β (Q27) Polyclonal Antibody-BS3603

Catenin-β (Q27) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3603-50, BS3603-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35222

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
IP: 1:50~1:200

Background: β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Catenin-β (Q27) polyclonal antibody detects endogenous levels of Catenin-β protein.

Bioworld Molecular Weight: ~ 92 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Catenin-β (Q27) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: 3T3-L1 whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Catenin-β (Q27) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysates using Catenin-β (Q27) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using Catenin-β (Q27) pAb.

Alternative Name: Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK/SW-cl.35; PRO2286

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Catenin-β.

Conjugate: Unconjugated

Modification: Unmodified