Catenin δ1 Polyclonal Antibody-BS90198

Catenin δ1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90198-50, BS90198-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60716 Human

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ICC

All Applications: WB:1:500
ICC:1:50-1:200

Background: This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.

Purification and Purity: Protein A purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: Catenin δ1 polyclonal antibody detects endogenous levels of Catenin δ1 protein.

Bioworld Molecular Weight: 108 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of delta 1 Catenin/CAS on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature., ICC staining delta 1 Catenin/CAS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: CAS, p120(ctn), CTNND1, KIAA0384, Cadherin associated Src substrate, Cadherin-associated Src substrate, CAS, Catenin (cadherin associated protein) delta 1, Catenin delta 1, Catenin delta, Catenin delta-1, CTND1, CTNND 1, CTNND, CTNND1, delta 1 Catenin, KIAA0384, p120, P120 CAS, p120 catenin, P120 CTN, p120(cas), p120(ctn), P120CAS, P120CTN antibody

Immunogen: Recombinant protein within human delta 1 Catenin/CAS aa 130-320.

Conjugate: Unconjugated

Modification: Unmodified