Product Description

Cathepsin B Activity Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F059-48, E-BC-F059-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Cathepsin B is a cysteine proteolytic enzyme in lysosomes. It is responsible for the hydrolysis, degradation and processing of proteins in organisms, thus maintaining a precise balance between protein synthesis and degradation. The abnormal proteolytic activity of cathepsin and the alteration of its expression pattern are involved in the key steps of the tumor disease process and can be used as markers for certain cancer types, serving as effective regulatory factors for cancer occurrence. As an important type of biomarker, the research on cathepsin is crucial for the study of drug targets and drug development.
The detection principle of this kit: Cathepsin B specifically cleaves the fluorescent substrate, and the fluorescent group of the product emits fluorescence. Fluorescence is detected at an excitation wavelength of 355 nm and an emission wavelength of 460 nm.

Research Areas: Amino Acids and Proteins

Result Type: Enzyme activity

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 0.009-0.351 U/L

Tested Sample Types: Animal tissue;Plant tissue;Cell

Assay Length: 30 min

Sensitivity: 0.009 U/L

Inter cv: 5.1%-9.9%

Intra cv: 2.1%-4.9%

Research Use Only