Product Description

Cathepsin B Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1869-50, ES1869-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors.

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CTSB, CPSB, Cathepsin B, APP secretase, APPS, Cathepsin B1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Cathepsin B. AA range:168-217

Isotype: IgG

Target: Cathepsin B

Specificity: Cathepsin B Polyclonal Antibody detects endogenous levels of Cathepsin B protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in the stratum spinosum of the epidermis, Weak expression is detected in the stratum granulosum,

Observed Band (KD): 38,40,25kD

Fields: Autophagy - animal, Lysosome, Apoptosis, Antigen processing and presentation, NOD-like receptor signaling pathway, Renin secretion

Function: Catalytic Activity: Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides
Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis
Similarity: Belongs to the peptidase C1 family
Subcellular Location: Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Subunit: Dimer of a heavy chain and a light chain cross-linked by a disulfide bond

Subcellular Location: Lysosome Melanosome Secreted, extracellular space Apical cell membrane, Peripheral membrane protein, Extracellular side Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: CTSB

Protein Name: Cathepsin B

Mouse Gene ID: 13030

Mouse SwissProt Number: P10605

Rat SwissProt Number: P00787

Research Use Only