Product Description

Cattle cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0100-48, ELK0100-96, ELK0100-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CMH2, cTnT, TnTc, Cardiomyopathy, Hypertrophic 2, Cardiac muscle troponin T, TNNT2, Troponin T Type 2, Cardiac

Reactivity: Cattle

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cardiovascular Biology

Assay Length: 3.5h

Sensitivity: 26.1 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

UniProt: P13789

Research Use Only