Product Description

Cattle EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK5971-48, ELK5971-96, ELK5971-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PK1, EGVEGF, PRK1, PROK1, Prokineticin 1, Black Mamba Toxin-Related Protein, Mambakine

Reactivity: Cattle

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cytokine, Tumor Immunity

Assay Length: 3.5h

Sensitivity: 6.3 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only