Product Description

Cattle FST (Follistatin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11440-48, ELK11440-96, ELK11440-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Cattle

Alternate Names: FST, Activin-binding protein,FS, FST, Follistatin

Sensitivity: 1.23 ng/mL

Assay Type: Sandwich

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids.

Assay Length: 3.5h

Research Area: Endocrinology, Reproductive Science, Hormone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle FST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle FST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle FST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle FST in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P50291

Research Use Only