Product Description

Cattle GnRH (Gonadotropin Releasing Hormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0603-48, ELK0603-96, ELK0603-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Cattle

Alternate Names: GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1

Sensitivity: 23.4 pg/mL

Assay Type: Competitive Inhibition

Standard: 4000 pg/mL

Detection Range: 62.50-4000 pg/mL

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 2.5h

Research Area: Endocrinology, Reproductive Science, Neuro Science, Genetic Science, Hormone Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only