Cattle IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit - ELK10693

Cattle IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10693-48, ELK10693-96, ELK10693-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BP4; HT29-IGFBP; IBP4

Assay Type: Competitive Inhibition

Sensitivity: 0.56 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Apoptosis; Tumor immunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q05716

Research Use Only