Product Description

Cattle IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7953-48, ELK7953-96, ELK7953-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Cattle

Alternate Names: IL-2RA,CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen, IL2Ra, Interleukin 2 Receptor Alpha

Sensitivity: 6.6 pg/mL

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Cd And Adhesion Molecule, Tumor Immunity, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only