Cattle IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7953-48, ELK7953-96, ELK7953-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: IL-2RA; CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 6.6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only