Product Description

Cattle PGFM (Prostaglandin F Metabolite) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0504-48, ELK0504-96, ELK0504-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PGFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PGFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PGFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PGFM in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 13, 14, Dihydro-15-keto-Prostaglandin F2

Reactivity: Cattle

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction

Assay Length: 3.5h

Sensitivity: 6.91 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only