Product Description

Cattle PGII (Pepsinogen II) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11840-48, ELK11840-96, ELK11840-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Cattle

Alternate Names: PGII

Sensitivity: 8.7 pg/mL

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, Plasma

Assay Length: 3.5h

Research Area: Enzyme And Kinase, Tumor Immunity, Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PGII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PGII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PGII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PGII in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only