Product Description

Cbl (phospho Tyr700) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7851-50, ES7851-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cbl proto-oncogene(CBL) Homo sapiens This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CBL, CBL2, RNF55, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CBL around the phosphorylation site of Tyr700. AA range:666-715

Isotype: IgG

Target: Cbl

Specificity: Phospho-Cbl (Y700) Polyclonal Antibody detects endogenous levels of Cbl protein only when phosphorylated at Y700.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Epithelium, T-cell,

Observed Band (KD): 120kD

Fields: ErbB signaling pathway, Ubiquitin mediated proteolysis, Endocytosis, Insulin signaling pathway, Bacterial invasion of epithelial cells, Pathways in cancer, Proteoglycans in cancer, Chronic myeloid leukemia

Function: Disease: Can be converted to an oncogenic protein by deletions or mutations that disturb its ability to down-regulate RTKs
Domain: The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain
Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme
Function: Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promo

Subcellular Location: Cytoplasm Cell membrane Cell projection, cilium Golgi apparatus Colocalizes with FGFR2 in lipid rafts at the cell membrane

Storage and Stability: -20°C/1 year

Gene Name: CBL

Protein Name: E3 ubiquitin-protein ligase CBL

Mouse Gene ID: 12402

Mouse SwissProt Number: P22682

Research Use Only