Product Description

Cbl (phospho-Y700) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4541-50, BS4541-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL , Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellμlar homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4, 11) or t(11, 14) abnormalities.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL, CBL, CBL2, RNF55

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Cbl around the phosphorylation site of Tyrosine 700.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Cbl (Y700) polyclonal antibody detects endogenous levels of Cbl only when phosphorylated at Tyr700.

Molecular Weight: ~ 100, 120kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P22681

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only