Product Description

CBP 35 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1881-50, ES1881-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: LGALS3, MAC2, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Galectin 3. AA range:141-190

Isotype: IgG

Target: Galectin-3

Specificity: CBP 35 Polyclonal Antibody detects endogenous levels of CBP 35 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: A major expression is found in the colonic epithelium, It is also abundant in the activated macrophages, Expressed in fetal membranes,

Observed Band (KD): 33kD

Function: Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis
Online Information: Galectin-3,Similarity: Contains 1 galectin domain
Subcellular Location: Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface
Subunit: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA
Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages

Subcellular Location: Cytoplasm Nucleus Secreted Secreted by a non-classical secretory pathway and associates with the cell surface Can be secreted, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10, it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059)

Storage and Stability: -20°C/1 year

Gene Name: LGALS3

Protein Name: Galectin-3

Mouse SwissProt Number: P16110

Rat Gene ID: 83781

Rat SwissProt Number: P08699

Research Use Only