Product Description

CD203c Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4004-50, ES4004-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Oct 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: ENPP3, PDNP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, Phosphodiesterase I beta, PD-Ibeta, Phosphodiesterase I/nucleotide pyrophosphatase 3, CD203c

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human ENPP3. AA range:281-330

Isotype: IgG

Target: CD203c

Specificity: CD203c Polyclonal Antibody detects endogenous levels of CD203c protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected on bile ducts in liver, and in blood serum (at protein level) (PubMed:15072822), Detected in prostate and uterus (PubMed:9344668), Detected on basophils, but not neutrophils (PubMed:11342463),

Observed Band (KD): 100kD

Fields: Purine metabolism, Pyrimidine metabolism, Starch and sucrose metabolism, Riboflavin metabolism, Nicotinate and nicotinamide metabolism, Pantothenate and CoA biosynthesis, Metabolic pathways, Nucleotide metabolism

Function: Catalytic Activity: A dinucleotide + H(2)O = 2 mononucleotides
Catalytic Activity: Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Cofactor: Binds 2 divalent metal cations per subunit
Enzyme Regulation: At low concentrations of ATP, a phosphorylated active site intermediate is formed which inhibits further ATP hydrolysis
Function: Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD
Induction: Up-regulated by stimulation by allergen or by cross-linking with IgE. The IgE-mediated activation is enhanced by tetradecanoyl phorbol acetate (TPA), a stimulator of the PKC pathway, and inhibited by the P13 kinase inhibitors, LY294002 and wortmannin. Up-regulated in invasive bile duct cancers
PTM: It has been suggested that the active SMB domain may be permitted co

Subcellular Location: Cell membrane, Single-pass type II membrane protein Apical cell membrane, Single-pass type II membrane protein Secreted Detected at the cell surface of basophils (PubMed:11342463) Detected at the apical plasma membrane of bile duct cells (PubMed:15072822) Located to the apical surface in intestinal and kidney epithelial cells Secreted in serum, and in lumen of epithelial cells

Storage and Stability: -20°C/1 year

Gene Name: ENPP3

Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Mouse Gene ID: 209558

Mouse SwissProt Number: Q6DYE8

Rat Gene ID: 54410

Rat SwissProt Number: P97675

Research Use Only