Product Description

CD227/MUC1 (Phospho-Tyr1229) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65397-50, BS65397-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: mucin 1, cell surface associated(MUC1) Homo sapiens This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellμlar signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellμlar localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC-p, IF/ICC, ELISA

Alternate Names: MUC1, PUM, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated ep

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: The antiserum was produced against synthesized peptide derived from human CD227/MUC1 around the phosphorylation site of Tyr1229. AA range:1201-1250

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity: Phospho-Mucin 1 (Y1229) Polyclonal Antibody detects endogenous levels of Mucin 1 protein only when phosphorylated at Y1229.

Molecular Weight: ~ 170 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15941

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only