Product Description

CD276 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4015-50, ES4015-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CD276, B7H3, PSEC0249, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CD276. AA range:271-320

Isotype: IgG

Target: CD276

Specificity: CD276 Polyclonal Antibody detects endogenous levels of CD276 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, Weakly expressed in resting monocytes, Expressed in dendritic cells derived from monocytes, Expressed in epithelial cells of sinonasal tissue, Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta,

Observed Band (KD): 100kd

Fields: Cell adhesion molecules

Function: Function: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling
Induction: By LPS in monocytes and by ionomycin in T and B lymphocytes. Up-regulated in cells mediating rejection of human tr

Subcellular Location: Membrane, Single-pass type I membrane protein

Storage and Stability: -20°C/1 year

Gene Name: CD276

Protein Name: CD276 antigen

Mouse Gene ID: 102657

Mouse SwissProt Number: Q8VE98

Rat Gene ID: 315716

Rat SwissProt Number: Q7TPB4

Research Use Only