Product Description

CD28 (phospho Tyr218) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8032-50, ES8032-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

Reactivity: Human

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CD28, T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220

Isotype: IgG

Target: CD28

Specificity: Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in T-cells and plasma cells, but not in less mature B-cells,

Observed Band (KD): 45kD

Fields: Cell adhesion molecules, T cell receptor signaling pathway, Intestinal immune network for IgA production, Type I diabetes mellitus, Measles, PD-L1 expression and PD-1 checkpoint pathway in cancer, Autoimmune thyroid disease, Systemic lupus erythematosus, Rheumatoid arthritis, Allograft rejection, Graft-versus-host disease, Viral myocarditis

Function: Function: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival
Online Information: CD28 entry,Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain
Subunit: Homodimer, disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70
Tissue Specificity: Expressed in T-cells and plasma cells, but not in less mature B-cells

Subcellular Location: Membrane, Single-pass type I membrane protein, [Isoform 3]: Cell surface

Storage and Stability: -20°C/1 year

Gene Name: CD28

Protein Name: T-cell-specific surface glycoprotein CD28

Mouse Gene ID: 12487

Mouse SwissProt Number: P31041

Research Use Only