Product Description

CD4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1909-50, ES1909-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CD4. AA range:401-450

Isotype: IgG

Target: CD4

Specificity: CD4 Polyclonal Antibody detects endogenous levels of CD4 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Highly expressed in T-helper cells, The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes, CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells,

Observed Band (KD): 51kD

Fields: Viral life cycle - HIV-1, Cytokine-cytokine receptor interaction, Cell adhesion molecules, Antigen processing and presentation, Hematopoietic cell lineage, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Yersinia infection, Human T-cell leukemia virus 1 infection, Human immunodeficiency virus 1 infection, PD-L1 expression and PD-1 checkpoint pathway in cancer, Primary immunodeficiency

Function: Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts
Miscellaneous: Primary receptor for HIV-1
Online Information: CD4 entry,PTM: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain
Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains
Subcellular Location: Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum
Subunit: Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to t

Subcellular Location: Cell membrane, Single-pass type I membrane protein Localizes to lipid rafts (PubMed:12517957, PubMed:9168119) Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum

Storage and Stability: -20°C/1 year

Gene Name: CD4

Protein Name: T-cell surface glycoprotein CD4

Mouse Gene ID: 12504

Mouse SwissProt Number: P06332

Research Use Only