Product Description

CD40 (phospho Thr254) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8067-50, ES8067-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIG

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD antigen CD40

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254. AA range:220-269

Isotype: IgG

Target: CD40

Specificity: Phospho-CD40 (T254) Polyclonal Antibody detects endogenous levels of CD40 protein only when phosphorylated at T254.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: B-cells and in primary carcinomas,

Observed Band (KD): 30kD

Fields: Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, Cell adhesion molecules, Toll-like receptor signaling pathway, Intestinal immune network for IgA production, Malaria, Toxoplasmosis, Human T-cell leukemia virus 1 infection, Epstein-Barr virus infection, Transcriptional misregulation in cancer, Asthma, Autoimmune thyroid disease, Systemic lupus erythematosus, Allograft rejection, Primary immunodeficiency, Viral myocarditis, Lipid and atherosclerosis

Function: Alternative Products: Additional isoforms seem to exist,Disease: Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3) [MIM: 606843]. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation
Function: Receptor for TNFSF5/CD40LG
Online Information: CD40 entry,Online Information: CD40 mutation db,Similarity: Contains 4 TNFR-Cys repeats
Subunit: Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6
Tissue Specificity: B-cells and in primary carcinomas

Subcellular Location: [Isoform I]: Cell membrane, Single-pass type I membrane protein, [Isoform II]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: CD40

Protein Name: Tumor necrosis factor receptor superfamily member 5

Mouse Gene ID: 21939

Mouse SwissProt Number: P27512

Research Use Only