Product Description

CD8B Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11787-50, ES11787-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: CD8B

Specificity: CD8B Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells, Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells, Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells,

Observed Band (KD): 23kD

Fields: Cell adhesion molecules, Antigen processing and presentation, Hematopoietic cell lineage, T cell receptor signaling pathway, Yersinia infection, Primary immunodeficiency

Function: Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing
Online Information: CD8 entry,PTM: Phosphorylated as a consequence of T-cell activation
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain
Subunit: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds

Subcellular Location: [Isoform 1]: Cell membrane, Single-pass type I membrane protein Requires the partner CD8A for efficient cell surface expression (PubMed:3145196) The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation, [Isoform 2]: Cell membrane, Single-pass type I membrane protein, [Isoform 3]: Secreted, [Isoform 4]: Cell membrane, Single-pass type I membrane protein, [Isoform 5]: Cell membrane, Single-pass type I membrane protein, [Isoform 6]: Secreted, [Isoform 7]: Secreted, [Isoform 8]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: CD8B CD8B1

Protein Name: T-cell surface glycoprotein CD8 beta chain (CD antigen CD8b)

Mouse SwissProt Number: P10300

Rat SwissProt Number: P05541

Research Use Only