Product Description

CDC42 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES10520-50, ES10520-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 80-160

Isotype: IgG

Target: CDC42

Specificity: CDC42 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Brain, Cajal-Retzius cell, Cervix, Embryo, Fetal brain, Fetal brain cortex, Placenta, Uter

Observed Band (KD): 21kD

Fields: MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Chemokine signaling pathway, Endocytosis, Axon guidance, VEGF signaling pathway, Focal adhesion, Adherens junction, Tight junction, T cell receptor signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Regulation of actin cytoskeleton, GnRH signaling pathway, Non-alcoholic fatty liver disease, AGE-RAGE signaling pathway in diabetic complications, Bacterial invasion of epithelial cells, Epithelial cell signaling in Helicobacter pylori infection, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection, Yersinia infection, Human papillomavirus infection, Pathways in cancer, Viral carcinogenesis, Proteoglycans in cancer, Renal cell carcinoma, Pancreatic cancer, Lipid and atherosclerosis

Function: Enzyme Regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia
Similarity: Belongs to the small GTPase superfamily. Rho family
Similarity: Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily
Subunit: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3,

Subcellular Location: Cell membrane, Lipid-anchor, Cytoplasmic side Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Midbody Cell projection, dendrite Localizes to spindle during prometaphase cells Moves to the central spindle as cells progressed through anaphase to telophase (PubMed:15642749) Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells (PubMed:15642749) Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1 (PubMed:15642749) Colocalizes with NEK6 in the centrosome (PubMed:20873783) In its active GTP-bound form localizes to the leading edge membrane of migrating dendritic cells (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: CDC42

Protein Name: Cell division control protein 42 homolog (G25K GTP-binding protein)

Mouse SwissProt Number: P60766

Rat SwissProt Number: Q8CFN2

Research Use Only