Product Description

CdcA7 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7716-50, ES7716-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: cell division cycle associated 7(CDCA7) Homo sapiens This gene was identified as a c-Myc responsive gene, and behaves as a direct c-Myc target gene. Overexpression of this gene is found to enhance the transformation of lymphoblastoid cells, and it complements a transformation-defective Myc Box II mutant, suggesting its involvement in c-Myc-mediated cell transformation. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CDCA7, JPO1, Cell division cycle-associated protein 7, Protein JPO1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CDCA7. AA range:141-190

Isotype: IgG

Target: CdcA7

Specificity: CdcA7 Polyclonal Antibody detects endogenous levels of CdcA7 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous with higher level in thymus and small intestine, Overexpressed in a large number of tumors, in blood from patients with acute myelogenous leukemia (AML) and in chronic myelogenous leukemia (CML) blast crisis,

Observed Band (KD): 43kD

Function: Function: Participates in MYC-mediated cell transformation, induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator
Induction: Activated by MYC and possibly E2F1
Miscellaneous: CDCA7 expression is correlated with MYC expression in lymphoblastoid, lymphoma and breast cancer cell lines
Tissue Specificity: Ubiquitous with higher level in thymus and small intestine. Overexpressed in a large number of tumors, in blood from patients with acute myelogenous leukemia (AML) and in chronic myelogenous leukemia (CML) blast crisis

Subcellular Location: Nucleus Cytoplasm Predominantly nuclear with some expression also seen in the cytoplasm Predominantly cytoplasmic when phosphorylated at Thr-163

Storage and Stability: -20°C/1 year

Gene Name: CDCA7

Protein Name: Cell division cycle-associated protein 7

Mouse Gene ID: 66953

Mouse SwissProt Number: Q9D0M2

Rat Gene ID: 311742

Rat SwissProt Number: Q4KM91

Research Use Only