Product Description

Cdk1/2/3 (phospho Thr14) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1285-50, ES1285-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: CDK1, CDC2, CDC28A, CDKN1, P34CDC2, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK2, CDKN2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 pr

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14. AA range:1-50

Isotype: IgG

Target: CDK1/CDC2

Specificity: Phospho-Cdk1/2/3 (T14) Polyclonal Antibody detects endogenous levels of Cdk1/2/3 protein only when phosphorylated at T14.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform 2 is found in breast cancer tissues,

Observed Band (KD): 34kD

Fields: Cell cycle, Oocyte meiosis, p53 signaling pathway, Cellular senescence, Gap junction, Progesterone-mediated oocyte maturation, Human immunodeficiency virus 1 infection, Viral carcinogenesis

Function: Catalytic Activity: ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Enzyme Regulation: Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it
Function: Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily
Similarity: Contains 1 protein kinase domain
Subunit: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with c

Subcellular Location: Nucleus Cytoplasm Mitochondrion Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Cytoplasmic during the interphase Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1 Accumulates in mitochondria in G2-arrested cells upon DNA-damage

Storage and Stability: -20°C/1 year

Gene Name: CDK1/CDK2/CDK3

Protein Name: Cyclin-dependent kinase 1/2/3

Mouse Gene ID: 12534/12566

Rat Gene ID: 54237

Rat SwissProt Number: P39951/Q63699

Research Use Only