Product Description

Cdk6 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8658-50, ES8658-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: cyclin dependent kinase 6(CDK6) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefS

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: Cyclin-dependent kinase 6 (EC 2.7.11.22, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE)

Clonality: Polyclonal

Immunogen: Synthetic peptide from human protein at AA range: 280-325

Isotype: IgG

Target: Cdk6

Specificity: The antibody detects endogenous Cdk6

Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed ubiquitously, Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas, Reduced levels in differentiating cells,

Observed Band (KD): 34kD

Fields: Cell cycle, p53 signaling pathway, PI3K-Akt signaling pathway, Cellular senescence, Cushing syndrome, Hepatitis C, Measles, Human cytomegalovirus infection, Influenza A, Human papillomavirus infection, Kaposi sarcoma-associated herpesvirus infection, Epstein-Barr virus infection, Pathways in cancer, Viral carcinogenesis, MicroRNAs in cancer, Pancreatic cancer, Glioma, Melanoma, Chronic myeloid leukemia, Small cell lung cancer, Non-small cell lung cancer, Breast cancer, Hepatocellular carcinoma

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Function: Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins
polymorphism: Genetic variations in CDK6 influences stature as a quantitative trait type 11 (STQTL11) [MIM: 612223]. Adult height is an easily observable and highly heritable complex continuous trait. Because of this, it is a model trait for studying genetic influence on quantitative traits
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily
Similarity: Contains 1 protein kinase domain

Subcellular Location: Cytoplasm Nucleus Cell projection, ruffle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Localized to the ruffling edge of spreading fibroblasts Kinase activity only in nucleus Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity) Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663)

Storage and Stability: -20°C/1 year

Gene Name: CDK6 CDKN6

Protein Name: Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE)

Mouse Gene ID: 12571

Mouse SwissProt Number: Q64261

Research Use Only