Product Description

Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K837-M-48, E-BC-K837-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.

Target Synonym: Cytochrome C Oxidase

Research Areas: Mitochondria Function;Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.79-47.0 U/L

Tested Sample Types: Cell

Assay Length: 35 min

Sensitivity: 0.79 U/L

Inter cv: 4.0-9.5%

Intra cv: 3.0-5.5%

Research Use Only