Product Description

Cellular Cuprous Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F102-48, E-BC-F102-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Abnormal accumulation of cellular cuprous in cells induces the oligomerization of key components of the lipoic acid-modified pyruvate dehydrogenase complex, which affects the tricarboxylic acid cycle, triggers proteotoxic stress, and induces cell death.
Cellular cuprous can catalyze the substrate to produce fluorescent substances, and the higher the copper ion concentration, the more fluorescent substances are generated per unit time. The fluorescence delete can be detected at the excitation wavelength of 395 nm and emission wavelength of 480 nm.

Research Areas: Cuproptosis

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Tested Sample Types: Cell

Assay Length: 50 min

Inter cv: 5.0-8.0%

Intra cv: 3.0-6.0%

Research Use Only